Microdissection-derived Murine Mcb Probes from Somatic Cell Hybrids
نویسندگان
چکیده
منابع مشابه
Somatic-cell hybrids producing antibodies against CEA.
Monoclonal antibodies to carcinoembryonic antigen (CEA) promise improved specificity for the measurement of this widely expressed human cancer antigen. A mouse monoclonal antibody binds weakly to CEA in perchloric acid extracts of tumour but strongly to CEA similarly isolated from serum, and its spectrum of cancer detection differs from conventional antisera.
متن کاملControlled Production of Proliferating Somatic Cell Hybrids
The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time. Sendai virus is used to promote cell hybridization. beta-propriolactone is employed as the inactivating agent of Sendai virus since it produces complete loss of viral infectivity while preserving viral fusion capacity. Cells are fused in monolaye...
متن کاملAmounts of Epstein-Barr virus DNA in somatic cell hybrids between burkitt lymphoma-derived cell lines.
The amounts of Epstein-Barr virus DNA in somatic cell hybrids between human lymphoid cell lines were found to be higher than in the parental cell lines, although the hybrids were not induced to spontaneous virus production.
متن کاملPredominance of the metastatic phenotype in somatic cell hybrids of the K-1735 murine melanoma.
The purpose of these studies was to determine whether the metastatic phenotype will dominate when metastatic and nonmetastatic clones of the K-1735 mouse melanoma are hybridized by somatic cell fusion. Three nonmetastatic and three metastatic clones were transfected with DNA from plasmids pSV2neo or pSV2hygro, which confer resistance to the drugs neomycin or hygromycin, respectively. The metast...
متن کاملPredominance of the Metastatic Phenotype in Somatic Cell Hybrids of the K-1735 Murine Melanoma1
The purpose of these studies was to determine whether the metastatic phenotype will dominate when metastatic and nonmetastatic clones of the K-173S mouse melanoma are hybridized by somatic cell fusion. Three nonmetastatic and three metastatic clones were transfected with DNA from plasmids pSV2neo or pS\2hygro, which confer resistance to the drugs neomycin or hygromycin, respectively. The metast...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2005
ISSN: 0022-1554,1551-5044
DOI: 10.1369/jhc.4b6598.2005